Physicochemical characterization, cytotoxic effect and toxicity evaluation of nanostructured lipid carrier loaded with eucalyptol

• DOI: 10.1186/s12906-021-03422-y
• Publication Date: 2021-10-07T10:23:19Z
• Authors: Mira Nadiah Mohd ...; Yazmin Hussin; Nurul Fattin Che ...; Muhammad Nazirul ...; Swee Keong Yeap; Heshu Sulaiman Ra...; Mas Jaffri Masarudin; Nurul Elyani Mohamad; Rasedee Abdullah; Noorjahan Banu Al...
• Keywords: Zeta potential; Dispersity; Eucalyptol

Eucalyptol is an active compound of eucalyptus essential oil and was reported to have many medical attributes including cytotoxic effect on breast cancer cells. However, it has low solubility in aqueous solutions which limits its bioavailability efficiency. In this study, nanostructured lipid carrier loaded with eucalyptol (NLC-Eu) formulated characterized the NLC-Eu towards cell lines determined. addition, toxicity animal model, BALB/c mice also incorporated into study validate safety NLC-Eu.Eucalyptol, a monoterpene oxide active, used formulate by using high pressure homogenization technique. The physicochemical characterization performed assess morphology, particle size, polydispersity index, zeta potential. vitro effects encapsulated human (MDA MB-231) murine (4 T1) were determined MTT assay. Additionally, acridine orange/propidium iodide assay conducted treated MDA MB-231 vivo sub-chronic prepared investigated model.As result, light, translucent, milky-colored showed size 71.800 ± 2.144 nm, poly-dispersity index 0.258 0.003, potential - 2.927 0.163 mV. Furthermore, TEM results displayed irregular round spherical morphology narrow distribution relatively uniformed particles. drug loading capacity entrapment efficiency 4.99 90.93%, respectively. exhibited both, mice, cells IC50 values 10.00 4.81 μg/mL 17.70 0.57 μg/mL, respectively at 72 h. induced apoptosis all studied did not show any signs toxicity, abnormality or mortality. Besides that, no significant changes observed body weight, internal organ hepatic renal histopathology, serum biochemistry, nitric malondialdehyde contents.This suggests that well-characterized offers safe promising system lines.