Comparative 3D genome architecture in vertebrates

• DOI: 10.1186/s12915-022-01301-7
• Publication Date: 2022-05-06T17:03:33Z
• Authors: Diyan Li; Mengnan He; Qianzi Tang; Shilin Tian; Jiaman Zhang; Yan Li; Danyang Wang; Long Jin; Chunyou Ning; Wei Zhu; Silu Hu; Keren Long; Jideng Ma; Jing Liu; Zhihua Zhang; Mingzhou Li
• Keywords: Euchromatin; Genomic Organization; Chromosome conformation capture; Comparative Genomics

Abstract Background The three-dimensional (3D) architecture of the genome has a highly ordered and hierarchical nature, which influences regulation essential nuclear processes at basis gene expression, such as transcription. While organization heterochromatin euchromatin can underlie differences in expression that determine evolutionary among species, way 3D is affected by forces within major lineages remains unclear. Here, we report comprehensive comparison genomes, using high resolution Hi-C data fibroblast cells fish, chickens, 10 mammalian species. Results This analysis shows correlation between size chromosome length affects territory (CT) upper hierarchy architecture, whereas lower features, including local transcriptional availability DNA, are selected through evolution vertebrates. Furthermore, conservation topologically associating domains (TADs) appears strongly associated with modularity profiles across Additionally, LINE SINE transposable elements likely contribute to organization, respectively, during architecture. Conclusions Our uncovers organizational features appear functional genes These findings guide ongoing investigations extending our understanding mechanisms shaping