Long non-coding RNA Tug1 modulates mitochondrial and myogenic responses to exercise in skeletal muscle

• DOI: 10.1186/s12915-022-01366-4
• Publication Date: 2022-07-18T12:03:38Z
• Authors: Adam J. Trewin; Jessica Silver; Hayley T. Dillon; Paul A. Della Gatta; Lewan Parker; Danielle S. Hiam; Yin Peng Lee; Mark Richardson; Glenn D. Wadley; Séverine Lamon
• Keywords: PPARGC1A

Abstract Background Mitochondria have an essential role in regulating metabolism and integrate environmental physiological signals to affect processes such as cellular bioenergetics response stress. In the metabolically active skeletal muscle, mitochondrial biogenesis is one important component contributing a broad set of adaptations occurring signals, which converge on transcriptional regulator peroxisome proliferator-activated receptor coactivator 1-alpha (PGC-1α), central beneficial effects exercise muscle. We investigated long non-coding RNA (lncRNA) taurine-upregulated gene 1 ( TUG1 ), interacts with PGC-1α responses Results human expression was upregulated post-exercise also positively correlated increase PPARGC1A ). Tug1 knockdown (KD) differentiating mouse myotubes led decreased Ppargc1a expression, impaired respiration morphology, enhanced myosin heavy chain slow isoform protein expression. Ca 2+ -mediated stimulus, KD prevented sequencing revealed that impacted transport genes several downstream targets. Finally, modulated ~300 were vitro model myotubes, including involved myogenesis. Conclusions found muscle after single session exercise, mechanistically, regulates networks associated calcium handling, differentiation These data demonstrate lncRNA exerts regulation over fundamental aspects biology stimuli.