Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane

• DOI: 10.1186/s12917-015-0531-5
• Publication Date: 2015-11-10T05:41:30Z
• Authors: Aline Ambrogi Fra...; Phelipe Oliveira ...; Luis Claudio Lope...; Raquel Yvonne Ara...; Maria Angelica Mi...; Durvanei Augusto ...
• Keywords: CD90; Homeobox protein NANOG; Amniotic stem cells; Stem cell marker

Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available humans, dogs, pigs, goats horses. Especially these have being considered promising candidates articular regeneration. Herein, we established characterized obtained equine SF SM. Samples were during arthroscopy cultured using MEM (Minimum Essential Medium). by morphology expression specific markers cells, pluripotency, inflammation, cell cycle. The medium was more effective (97 % ± 2) maintain both cultures. cultures composed adherent with fibroblast-like shape, which had a growth pattern represented sigmoidal curve. After expansion, analyzed flow cytometry inflammatory, cycle markers, lineages showed significant CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, LY6a. contrast, differences phases between lineages, not observed relation mitochondrial electrical potential. Given large impact that joint pathology on athletic performance horses, our results suggested SM are sources satisfactory characteristics gene can be used regenerative medicine.