Biomarkers of oxidative stress in saliva in pigs: analytical validation and changes in lactation

• DOI: 10.1186/s12917-019-1875-z
• Publication Date: 2019-05-14T11:03:52Z
• Authors: Camila Peres Rubio; Eva Mainau; José Joaquín Cerón; Maria Dolores Con...; Silvia Martínez-S...; Elena Navarro; Fernando Tecles; Xavier Manteca; Damian Escribano
• Keywords: Oxidant; 0301 basic medicine; 2. Zero hunger; Efectes de l'estrès sobre els animals; Veterinary medicine; Sus scrofa; Hydrogen Peroxide; 04 agricultural and veterinary sciences; Antioxidants; Uric Acid; 0403 veterinary science; Oxidative Stress; 03 medical and health sciences; Porcs; Advanced Oxidation Protein Products; SF600-1100; Sows; Animals; Lactation; Female; Antioxidant; Reactive oxygen species; Saliva; Biomarkers; Research Article

Biomarkers of oxidative stress in pigs have been measured serum/plasma samples. However, blood collection can be highly stressful to the animals. Saliva is a biological fluid with several advantages over blood, since it easily collected without animals, being therefore an ideal sample this species. The objective study was validation assays for evaluation status saliva pigs. For purpose, three commonly used evaluate total antioxidant capacity (TAC): trolox equivalent (TEAC), cupric reducing (CUPRAC), and ferric ability plasma (FRAP)), one individual (uric acid) two oxidant concentrations (advanced oxidation protein products (AOPP) hydrogen peroxide (H2O2)) were validated porcine saliva. In addition, possible changes these sows' during lactation studied. methods had intra- inter-assays coefficient variation lower than 15%. They also showed adequate linearity recovery, their detection limits low enough detect analytes Overall analytical tests that our are valid reliable observed salivary biomarkers change situation such as sows. All evaluated demonstrated high accuracy imprecision. has significant Therefore, opens new possibility using non-invasive