Early-life house dust mite aeroallergen exposure augments cigarette smoke-induced myeloid inflammation and emphysema in mice

• DOI: 10.1186/s12931-024-02774-6
• Publication Date: 2024-04-13T18:02:13Z
• Authors: Nok Him Fung; Quynh Anh Nguyen; Catherine Owczarek; Nick Wilson; Nadeem Elahee Doomun; David De Souza; Kylie Quinn; Stavros Selemidis; Jonathan McQualter; Ross Vlahos; Hao Wang; Steven Bozinovski
• Keywords: ACO; Emphysema; Inflammation; 0301 basic medicine; RC705-779; Macrophages; Research; Pyroglyphidae; 610; Asthma; Cigarette Smoking; Mice, Inbred C57BL; Diseases of the respiratory system; Mice; 03 medical and health sciences; Pulmonary Emphysema; Hypersensitivity; COPD; Humans; Animals; MMP-12

Abstract Background Longitudinal studies have identified childhood asthma as a risk factor for obstructive pulmonary disease (COPD) and asthma-COPD overlap (ACO) where persistent airflow limitation can develop more aggressively. However, a causal link between childhood asthma and COPD/ACO remains to be established. Our study aimed to model the natural history of childhood asthma and COPD and to investigate the cellular/molecular mechanisms that drive disease progression.

Methods Allergic airways disease was established in three-week-old young C57BL/6 mice using house dust mite (HDM) extract. Mice were subsequently exposed to cigarette smoke (CS) and HDM for 8 weeks. Airspace enlargement (emphysema) was measured by the mean linear intercept method. Flow cytometry was utilised to phenotype lung immune cells. Bulk RNA-sequencing was performed on lung tissue. Volatile organic compounds (VOCs) in bronchoalveolar lavage-fluid were analysed to screen for disease-specific biomarkers.

Results Chronic CS exposure induced emphysema that was significantly augmented by HDM challenge. Increased emphysematous changes were associated with more abundant immune cell lung infiltration consisting of neutrophils, interstitial macrophages, eosinophils and lymphocytes. Transcriptomic analyses identified a gene signature where disease-specific changes induced by HDM or CS alone were conserved in the HDM-CS group, and further revealed an enrichment of Mmp12, Il33 and Il13, and gene expression consistent with greater expansion of alternatively activated macrophages. VOC analysis also identified four compounds increased by CS exposure that were paradoxically reduced in the HDM-CS group.

Conclusions Early-life allergic airways disease worsened emphysematous lung pathology in CS-exposed mice and markedly alters the lung transcriptome.