Vascular endothelial microparticles-incorporated microRNAs are altered in patients with diabetes mellitus

• DOI: 10.1186/s12933-016-0367-8
• Publication Date: 2016-03-22T08:33:48Z
• Authors: Felix Jansen; Han Wang; David Przybilla; Bernardo S. Franklin; Andreas Dolf; Philipp Pfeifer; Theresa Schmitz; Anna Flender; Elmar Endl; Georg Nickenig; Nikos Werner
• Keywords: Angiology

Circulating microRNAs (miRs) are differentially regulated and selectively packaged into microparticles (MPs). We evaluated whether diabetes mellitus alters circulating vascular endothelial MP-incorporated miRs expression levels. MPs were isolated from 135 patients with or without type II characterized using flow cytometer electron microscope. Nine involved in the regulation of performance—miR-126, miR-222, miR-let7d, miR-21, miR-30, miR-92a, miR-139, miR-199a miR-26a—were quantified by reverse transcription polymerase chain reaction. Among those, miR-126 miR-26a significantly reduced diabetic compared to non-diabetic patients. Patients low levels at higher risk for a concomitant coronary artery disease. MP-sorting experiments showed that cells major cell sources containing miR-26a, respectively. Finally, accordance our clinical results, vitro revealed hyperglycemia reduces packaging EMPs. Diabetes potential implications on heath.