Delivery of a functional anti-trypanosome Nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius

• DOI: 10.1186/s12934-014-0156-6
• Publication Date: 2014-11-14T12:34:27Z
• Authors: Linda De Vooght; Guy Caljon; Karin De Ridder; Jan Van Den Abbeele
• Keywords: Tsetse fly; Symbiotic bacteria

Sodalis glossinidius, a vertically transmitted microbial symbiont of the tsetse fly, is currently considered as potential delivery system for anti-trypanosomal components that reduce or eliminate capability fly host to transmit parasitic trypanosomes, an approach also known paratransgenesis. An essential step in developing paratransgenic stable colonization adult flies and their progeny with recombinant bacteria, expressing trypanocidal effector molecules tissues where parasite resides. In this study, was tested its ability deliver functional anti-trypanosome nanobodies (Nbs) Glossina morsitans morsitans. We characterized vitro vivo stability (recSodalis) potent trypanolytic nanobody, i.e. Nb_An46. show recSodalis competitive WT conditions transiently cleared endogenous population can be successfully repopulated at high densities. addition, vertical transmission offspring observed. Finally, we demonstrated expressed significant levels (ng range) Nb_An46 different tissues, including midgut important developmental stage trypanosome occurs. proof-of-concept genetically engineered express release amounts Nbs fly. The application innovative concept using pathogen-targeting delivered by insect symbiotic bacteria could extended other vector-pathogen systems.