Development of a multi-gene expression system in Xanthophyllomyces dendrorhous

• DOI: 10.1186/s12934-014-0175-3
• Publication Date: 2014-12-03T11:46:47Z
• Authors: Kiyotaka Y Hara; Toshihiko Morita; Masao Mochizuki; Keisuke Yamamoto; Chiaki Ogino; Michihiro Araki; Akihiko Kondo
• Keywords: Expression (computer science)

Red yeast, Xanthophyllomyces dendrorhous (Phaffia rhodozyma) is the only yeast known to produce astaxanthin, an anti-oxidant isoprenoid (carotenoid) that widely used in aquaculture, food, pharmaceutical and cosmetic industries. Recently, potential of this microorganism as a platform cell factory for production has been recognized because high flux through its native terpene pathway. Addition mevalonate, common precursor biosynthesis, shown be critical enhance astaxanthin content X. dendrorhous. However, addition mevalonate unrealistic during industrial it unstable costly chemical. Therefore, up-regulating intracellular supply by enhancing synthetic pathway though genetic engineering promising strategy improve system strongly express multiple genes poorly developed Here, we gene expression using plasmids containing three strong promoters (actin, alcohol dehydrogenase triose-phosphate isomerase) their terminators. Using system, encoding acetoacetyl-CoA thiolase, HMG-CoA synthase reductase were overexpressed at same time. This triple overexpressing strain showed increase compared with each single strain. Additionally, overexpression together involved β-carotene synthesis synergetic effect on increasing production. Finally, was enhanced 2.1-fold parental without reduction growth. We overexpress substrate enhanced, causing Combining overproduces various isoprenoids