Condition-specific promoter activities in Saccharomyces cerevisiae

• DOI: 10.1186/s12934-018-0899-6
• Publication Date: 2018-04-19T11:01:16Z
• Authors: Liang Xiong; Yu Zeng; Rui-Qi Tang; Hal S. Alper; Feng-Wu Bai; Xin-Qing Zhao
• Keywords: 0301 basic medicine; 0303 health sciences; Hot Temperature; Saccharomyces cerevisiae Proteins; Xylose; Research; Green Fluorescent Proteins; Synthetic promoter; Transcription regulation; Environmental stress; Saccharomyces cerevisiae; Microbiology; QR1-502; 3. Good health; Industrial Microbiology; 03 medical and health sciences; Stress, Physiological; Biofuels; Gene Expression Regulation, Fungal; Fermentation; Furaldehyde; Promoter strength; Promoter Regions, Genetic; Acetic Acid

Saccharomyces cerevisiae is widely studied for production of biofuels and biochemicals. To improve efficiency under industrially relevant conditions, coordinated expression multiple genes by manipulating promoter strengths an efficient approach. It known that gene highly dependent on the practically used environmental conditions subject to dynamic changes. Therefore, investigating activities S. different culture in time points, especially stressful great importance. In this study, various promoters presence xylose were characterized using yeast enhanced green fluorescent protein (yEGFP) as a reporter. The stresses include toxic levels acetic acid furfural, high temperature, which are related fermentation lignocellulosic hydrolysates. addition eight native promoters, synthetic hybrid P3xC-TEF1 was also evaluated. results revealed P TDH3 showed highest almost all conditions. Importantly, these two exhibited stabilities throughout cultivation. However, ADH1 PGK1 , generally regarded 'constitutive' decreased significantly certain suggesting cautions should be taken use such constitutive drive Interestingly, HSP12 HSP26 able response both temperature stress. Moreover, led moderate yEGFP when sole carbon source, indicating could inducing proper utilization. here changes batch inhibitors well xylose. These provide insights selection construct strains bioproduction practical Our encouraged applications with stability strain development.