A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

• DOI: 10.1186/s12934-021-01691-3
• Publication Date: 2021-10-18T17:49:23Z
• Authors: Jiliang Deng; Yanling Wu; Zhaohui Zheng; Nanzhu Chen; Xiaozhou Luo; Hongting Tang; Jay D. Keasling
• Keywords: Synthetic Biology

Saccharomyces cerevisiae is an important synthetic biology chassis for microbial production of valuable molecules. Promoter engineering has been frequently applied to generate more promoters with a variety defined characteristics in order achieve well-regulated genetic network high efficiency. Galactose-inducible (GAL) expression systems, composed GAL and multiple regulators, have widely used protein overexpression pathway construction S. cerevisiae. However, the function each element how they interact regulators are not well known.Here, library demonstrate that upstream activating sequences (UASs) core synergistic relationship determines performance promoter under different carbon sources. We found strengths could be fine-tuned by manipulating sequence, number, substitution UASs. Core replacement generated twofold strength improvement compared GAL1 sources strain GAL80 engineering. These results represent expansion classic system increased dynamic range good tolerance sources.In this study, effect on evaluated series well-controlled constructed. By studying interaction created.