PHB production from cellobiose with Saccharomyces cerevisiae

• DOI: 10.1186/s12934-022-01845-x
• Publication Date: 2022-06-21T09:03:11Z
• Authors: Anna Ylinen; Jorg C. de Ruijter; Paula Jouhten; Merja Penttilä
• Keywords: Cupriavidus necator; Arabitol; Pentose

Replacement of petrochemical-based materials with microbially produced biodegradable alternatives calls for industrially attractive fermentation processes. Lignocellulosic offer non-edible cultivated sugars, but require often use expensive sugar releasing enzymes, such as β-glucosidases. These cellulose treatment costs could be reduced if microbial production hosts short cellodextrins cellobiose directly their substrates. In this study, we demonstrate poly(hydroxybutyrate) (PHB) in yeast Saccharomyces cerevisiae using a sole carbon source. Yeast strains expressing PHB pathway genes from Cupriavidus necator and cellodextrin transporter gene CDT-1 Neurospora crassa were complemented either β-glucosidase GH1-1 N. or phosphorylase cbp Ruminococcus flavefaciens. utilization routes Gh1-1 Cbp enzymes differ energetics dynamics. However, both enabled higher per consumed accumulation % cell dry weight (CDW) than glucose As expected, the faster Cbp, flask bioreactor batch cultures. shake flasks, final CDW was reached route (10.0 ± 0.3%) (8.1 0.2%). achieved better aerated pH-controlled bioreactors, comparison to relative performance switched. notable levels 13.4 0.9% 18.5 3.9% routes, respectively. The average molecular weights accumulated similar routes; approximately 500 kDa 450 genes, formation high weights, combined efficient conversion, demonstrates highly potential solution improving attractiveness sustainable polymer cells.