SKA3 promotes cell proliferation and migration in cervical cancer by activating the PI3K/Akt signaling pathway

• DOI: 10.1186/s12935-018-0670-4
• Publication Date: 2018-11-14T13:57:06Z
• Authors: Rong Hu; Ming-qing Wang; Wen-bo Niu; Yan-jing Wang; Yang-yang Liu; Ling-yu Liu; Ming Wang; Juan Zhong; Hai-yan You; Xiao-hui Wu; Ning Deng; Lu Lu; Lian-bo Wei
• Keywords: HeLa; Cyclin B1

Cervical cancer (CC) is one of the most common cancers among females worldwide. Spindle and kinetochore-associated complex subunit 3 (SKA3), located on chromosome 13q, was identified as a novel gene involved in promoting malignant transformation cancers. However, function underlying mechanisms SKA3 CC remain unknown. Using Oncomine database, we found that expression mRNA higher tissues than normal linked with poor prognosis. In our study, immunohistochemistry showed increased tissues. The effect cell proliferation migration evaluated by CCK8, clone formation, Transwell wound-healing assays HeLa SiHa cells stable overexpression knockdown. addition, established xenograft tumor model vivo. promoted accelerated growth. We further regulating cycle progression PI3K/Akt signaling pathway via RNA-sequencing (RNA-Seq) set enrichment analyses. Western blotting results revealed levels p-Akt, cyclin E2, CDK2, D1, CDK4, E2F1 p-Rb cells. Additionally, use an Akt inhibitor (GSK690693) significantly reversed capacity induced suggest contributes to growth activating PI3K–Akt pathway, which may provide potential therapeutic targets for treatment.