Two circPPFIA1s negatively regulate liver metastasis of colon cancer via miR-155-5p/CDX1 and HuR/RAB36

• DOI: 10.1186/s12943-022-01667-w
• Publication Date: 2022-10-12T04:02:52Z
• Authors: Haein Ji; Tae Won Kim; Woo Joo Lee; Seong Dong Jeong; Yong Beom Cho; Hyeon Ho Kim
• Keywords: Liver Cancer

Circular RNAs (circRNAs) play a critical role in colorectal cancer (CRC) progression, including metastasis. However, the detailed molecular mechanism is not fully understood.Differentially expressed circRNAs between primary KM12C and liver metastatic KM12L4 colon cells were identified by microarray. The expression of was measured semi-quantitative (semi-qPCR) real time-quantitative PCR (RT-qPCR). Metastatic potential invasive migratory abilities, metastasis examined transwell assays intrasplenic injection, respectively. CircPPFIA1-associated microRNA (miRNA) RNA-binding protein (RBP) screened an antisense oligonucleotide (ASO) pulldown experiment. effects circPPFIA1 on target gene evaluated RT-qPCR western blot analyses.By analyzing circRNA microarray data, we two anti-metastatic generated from PPFIA1 with different length, which named circPPFIA1-L (long) -S (short). They significantly downregulated compared to cells. knockdown circPPFIA1s enhanced increased Conversely, overexpression weakened inhibited found function as sponges oncogenic miR-155-5p Hu antigen R (HuR) ASO upregulated tumor-suppressing CDX1 conversely RAB36 decoying sequestering HuR, respectively.Our findings demonstrate that inhibit CRC via miR-155-5p/CDX1 HuR/RAB36 pathways.