Induction of omega 6 inflammatory pathway by sodium metabisulfite in rat liver and its attenuation by ghrelin

• DOI: 10.1186/s12944-015-0008-3
• Publication Date: 2015-02-16T20:28:15Z
• Authors: Sevim Ercan; Ceren Kencebay; Goksun Basaranlar; Filiz Ozcan; Narin Derin; Mutay Aslan
• Keywords: Sodium metabisulfite; Docosapentaenoic acid

Sodium metabisulfite is commonly used as preservative in foods but can oxidize to sulfite radicals initiating molecular oxidation. Ghrelin a peptide hormone primarily produced the stomach and has anti-inflammatory effects many organs. This study aimed assess endogenous omega-3 (n-3) omega-6 (n-6) polyunsaturated fatty acids (PUFAs) rat peripheral organs following sodium treatment determine possible effect of ghrelin on changes n-6 inflammatory pathway.Male Wistar rats included were allowed free access standard chow. was given by gastric gavage administered intraperitoneally for 5 weeks. Levels arachidonic acid (AA, C20:4n-6), dihomo-gamma-linolenic (DGLA, C20:3n-6), eicosapentaenoic (EPA, C20:5n-3) docosahexaenoic (DHA, C22:6n-3) liver, heart kidney tissues determined an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Cyclooxygenase (COX) prostaglandin E2 (PGE2) measured tissue samples evaluate pathway.Omega-6 PUFA levels, AA/DHA AA/EPA ratio significantly increased liver compared controls. No significant change observed levels. Tissue activity COX PGE2 levels also treated decreased reduced rats.Current results suggest that exerts action through modulation hepatic tissue.