MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α

• DOI: 10.1186/s12950-018-0191-x
• Publication Date: 2018-08-20T09:57:32Z
• Authors: Li Xu; Wen J. Sun; Ai J. Jia; Lu L. Qiu; Bing Xiao; Lin Mu; Jian M. Li; Xiu F. Zhang; Yan Wei; Cong Peng; Dong S. Zhang; Xu D. Xiang
• Keywords: 0301 basic medicine; T helper 17 cells; 03 medical and health sciences; Neutrophils-dominant asthma; Research; Therapeutics. Pharmacology; RM1-950; Methtyl-CpG binding domain protein 2; Hypoxia inducible factor-1α; 3. Good health

T helper 17 (Th17) cells have proven to be crucial in the pathogenesis of neutrophils-dominant asthma. Hypoxia inducible factor-1α (HIF-1α) is involved allergic responses Our previous studies indicated that Methtyl-CpG binding domain protein 2 (MBD2) expression was increased asthma patients. The aim present study understand how MBD2 interacts with HIF-1α regulate Th17 cell differentiation and IL-17 asthma.A mouse model established using female C57BL/6 mice investigate regulation flow cytometry, western blot or qRT-PCR. genes were silenced overexpressed through lentiviral transduction explore roles release neutrophilic inflammatory phenotype successfully. This characterized by airway hyperresponsiveness (AHR), BALF neutrophil granulocytes, activated differentiation, high levels. significantly lung spleen Through overexpression silencing genes, we concluded secretion. Moreover, also found expression.Our findings uncovered new for HIF-1α, provide novel insights into epigenetic