Polymer coated gold-ferric oxide superparamagnetic nanoparticles for theranostic applications

• DOI: 10.1186/s12951-018-0405-7
• Publication Date: 2018-10-13T00:59:47Z
• Authors: Muhammad Raisul A...; Siddesh Umapathi; Harika Mahendrakar; Tunyaboon Laemthong; Holly Coleman; Denise Muchangi; Santimukul Santra; Manashi Nath; Sutapa Barua
• Keywords: Superparamagnetism; Surface Modification; Iron oxide nanoparticles

Engineered inorganic nanoparticles (NPs) are essential components in the development of nanotechnologies. For applications nanomedicine, particles need to be functionalized ensure a good dispersibility biological fluids. In many cases however, functionalization is not sufficient: become either coated by corona serum proteins or precipitate out solvent. We show that changing coating magnetic iron oxide NPs using poly-L-lysine (PLL) polymer colloidal stability dispersion improved aqueous solutions including water, phosphate buffered saline (PBS), PBS with 10% fetal bovine (FBS) and cell culture medium, internalization toward living mammalian cells profoundly affected.A multifunctional NP designed perform near-infrared (NIR)-responsive remote control photothermal ablation for treatment breast cancer. contrast previously reported studies gold (Au) (Fe3O4) core-shell NPs, Janus-like nanostructure synthesized Fe3O4 decorated Au resulting an approximate size 60 nm mean diameter. The surface trisoctahedral Au-Fe3O4 was positively charged polymer, PLL deliver inside cells. PLL-Au-Fe3O4 were characterized transmission electron microscopy (TEM), XRD, FT-IR dynamic light scattering (DLS). unique properties both plasmon resonance superparamagnetic moment result multimodal platform use as nanothermal ablator also imaging (MRI) agent, respectively. Taking advantage therapy, incubated BT-474 MDA-MB-231 cancer cells, investigated cytotoxicity intracellular uptake, remotely triggered NIR laser ~ 808 (1 W/cm2 10 min).The increased robustness (PLL-Au-Fe3O4) media serum. It revealed no significant (< 10%) induced itself at concentrations up 100 μg/ml. Brightfield microscopy, fluorescence TEM showed uptake exhibited 40 60% inhibition growth, respectively following internalized μg/ml NPs. treated but without < death compared CONCLUSION: Combined together, results demonstrate new integrates diagnostics function tumors into single nanoplatform exhibiting death.