Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles

• DOI: 10.1186/s12951-022-01401-0
• Publication Date: 2022-04-27T10:04:21Z
• Authors: Ruijie Qian; Kun Wang; Yawen Guo; Hongyan Li; Ziyang Zhu; Xiaojuan Huang; Chengpeng Gong; Yu Gao; Rong Guo; Biao Yang; Chenyang Wang; Dawei Jiang; Xiaoli Lan; Rui An; Zairong Gao
• Keywords: Nanocarriers; Sonodynamic therapy

Abstract Background Photodynamic therapy (PDT) is a promising antitumor strategy with fewer adverse effects and higher selectivity than conventional therapies. Recently, series of reports have suggested that PDT induced by Cerenkov radiation (CR) (CR-PDT) has deeper tissue penetration traditional PDT; however, the coupling radionuclides photosensitizers may cause severe side effects. Methods We designed tumor-targeting nanoparticles ( 131 I-EM@ALA) loading 5-aminolevulinic acid (ALA) into an I-labeled exosome mimetic (EM) to achieve combined therapy. In addition playing radiotherapeutic role, I served as internal light source for (CR). Results The drug-loaded effectively targeted tumors confirmed confocal imaging, flow cytometry, small animal fluorescence imaging. vitro in vivo experiments demonstrated I-EM@ALA produced effect through synergy radiotherapy CR-PDT. killed tumor cells inducing DNA damage activating lysosome-mitochondrial pathways. No obvious abnormalities hematology analyses, blood biochemistry, or histological examinations were observed during treatment. Conclusions successfully engineered nanocarrier coloaded radionuclide photosensitizer precursor treatment breast cancer. Graphical