The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling

• DOI: 10.1186/s12951-024-02404-9
• Publication Date: 2024-04-10T16:01:45Z
• Authors: Nan Li; Jingnan Huang; Shangwen He; Qiaocong Zheng; Feng Ye; Zhengxing Qin; Dong Wang; Ting Xiao; Mengyuan Mao; Zhenhua Zhou; Tingxi Tang; Longshan Zhang; Xiaoqing Wang; Yingqiao Wang; Ying Lyu; Laiyu Liu; Lingyun Dai; Jigang Wang; Jian Guan
• Keywords: Proteome; Posttranslational modification

Plasma proteins are considered the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based proteomics has been applied to identify in plasma, but complexity plasma proteome extremely large dynamic range protein abundances make clinical application highly challenging. We designed synthesized zeolite-based nanoparticles deplete high-abundance proteins. The resulting novel proteomic assay can measure approximately 3000 a 45 min chromatographic gradient. Compared those neat depleted identified by our exhibited distinct biological profiles, as validated several public datasets. A pilot investigation profile hepatocellular carcinoma (HCC) cohort 15 promising features, highlighting diagnostic value distinguishing individuals with without HCC. Furthermore, this be easily integrated all current downstream profiling methods potentially extended other biofluids. In conclusion, we established robust efficient unprecedented identification depth, paving way translation into applications.