An end-to-end approach for single-cell infrared absorption spectroscopy of bacterial inclusion bodies: from AFM-IR measurement to data interpretation of large sample sets

• DOI: 10.1186/s12951-024-02674-3
• Publication Date: 2024-07-10T06:02:41Z
• Authors: Wouter Duverger; Grigoria Tsaka; Ladan Khodaparast; Laleh Khodaparast; Nikolaos Louros; Frederic Rousseau; Joost Schymkowitz
• Keywords: Inclusion Bodies; 0303 health sciences; Spectrophotometry, Infrared; Research; Microscopy, Atomic Force; Image analysis; 03 medical and health sciences; AFM-IR; Medical technology; Escherichia coli; Protein aggregation; R855-855.5; Single-Cell Analysis; Infrared spectroscopy; TP248.13-248.65; Biotechnology

Inclusion bodies (IBs) are well-known subcellular structures in bacteria where protein aggregates collected. Various methods have probed their structure, but single-cell spectroscopy remains challenging. Atomic Force Microscopy-based Infrared Spectroscopy (AFM-IR) is a novel technology with high potential for the characterisation of biomaterials such as IBs.