Klotho negatively regulated aerobic glycolysis in colorectal cancer via ERK/HIF1α axis

• DOI: 10.1186/s12964-018-0241-2
• Publication Date: 2018-06-08T08:15:36Z
• Authors: Qingguo Li; Yaqi Li; Lei Liang; Jing Li; Dakui Luo; Qi Liu; Sanjun Cai; Xinxiang Li
• Keywords: Anaerobic glycolysis

Klotho (KL) was originally characterized as an aging suppressor gene, and has been identified a tumor gene in variety of cancers, including colorectal cancer. Recent years have witnessed the importance metabolism transformation cancer cell malignancies maintenance. Aberrant is considered to be hallmark Our previous studies demonstrated that KL played negative roles colon proliferation metastasis. However, its role reprogramming seldom reported. The aim this study examine aerobic glycolysis Combining maximum standardized uptake value (SUVmax), which obtained preoperatively via PET/CT scan, with immunohistochemistry staining, we analyzed correlation between SUVmax expression tissues. impact on glucose mechanisms were further validated vitro vivo. Patients lower exhibited higher 18F-FDG (P < 0.05), indicating might participate regulation. In assay by using lines supported observation. By overexpressing HTC116 SW480 cells, observed inhibited mitochondrial respiration increased, regulator glycolysis. To seek for underlying mechanisms, tried dig out relation HIF1α signaling pathway, found negatively regulated protein level transcriptional activity. Western blot analysis showed overexpression ERK part through regulation ERK/ axis. Taken together,