LOXL1 modulates the malignant progression of colorectal cancer by inhibiting the transcriptional activity of YAP

• DOI: 10.1186/s12964-020-00639-1
• Publication Date: 2020-09-10T18:03:52Z
• Authors: Lin Hu; Jing Wang; Yunliang Wang; Linpeng Wu; Chao Wu; Bo Mao; E. Maruthi Prasad; Yuhong Wang; Y. Eugene Chin
• Keywords: Tumor progression

LOX-like 1 (LOXL1) is a lysyl oxidase, and emerging evidence has revealed its effect on malignant cancer progression. However, role in colorectal (CRC) the underlying molecular mechanisms have not yet been elucidated.LOXL1 expression was detected by immunohistochemistry, western blotting real-time PCR. In vitro, colony formation, wound healing, migration invasion assays were performed to investigate effects of LOXL1 cell proliferation, invasion. vivo, metastasis models mouse xenografts used assess tumorigenicity ability. Molecular biology experiments utilized reveal which modulates Hippo pathway.LOXL1 highly expressed normal colon tissues compared with tissues. silencing CRC lines dramatically enhanced migration, invasion, while overexpression exerted opposite effects. The results vivo demonstrated that drastically inhibited metastatic progression tumour growth. Mechanistically, transcriptional activity Yes-associated protein (YAP) interacting MST1/2 increasing phosphorylation MST1/2.LOXL1 may function as an important suppressor regulating growth, via negative regulation YAP activity. Video abstract.