The role of the globular heads of the C1q receptor in HPV-16 E2-induced human cervical squamous carcinoma cell apoptosis via a mitochondria-dependent pathway

• DOI: 10.1186/s12967-014-0286-y
• Publication Date: 2014-10-04T11:10:51Z
• Authors: Zheng-lin Chen; Ya-juan Su; Hui-lin Zhang; Ping-qing Gu; Ling-juan Gao
• Keywords: Squamous carcinoma

Human papillomavirus type-16 (HPV-16) E2 protein acts as a transcriptional modulator and plays key role in regulating many biological responses. The purpose of this study was to investigate the relationship between HPV-16 E2, receptor for globular heads human C1q (gC1qR) gene expression, mitochondrial dysfunction apoptosis regulation cervical squamous carcinoma cells (C33a SiHa).HPV-16 gC1qR expression examined using real-time PCR western blot analysis. Apoptosis C33a SiHa assessed by flow cytometry. Mitochondrial function detected via ROS generation, amount cytosolic Ca2+, changes membrane potential (Δψm).The significantly decreased samples relative non-cancerous cervix samples. that were transfected with vector encoding displayed increased well an up-regulation cellular apoptosis, which abrogated addition small-interfering RNA (siRNA).These data support mechanism whereby important E2-induced cell mitochondria-dependent pathway.