Plasma interleukin-37 is increased and inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells in systemic juvenile idiopathic arthritis patients

• DOI: 10.1186/s12967-018-1655-8
• Publication Date: 2018-10-11T00:35:49Z
• Authors: Miao Feng; Min Kang; Feng He; Zonghui Xiao; Zhewei Liu; Hailan Yao; Jianxin Wu
• Keywords: Proinflammatory cytokine; Interleukin 18

Interleukin (IL)-37 has emerged as a novel anti-inflammatory cytokine that play an immunosuppressive role in regulating inflammatory response. This study aimed to measure IL-37 levels the plasma and peripheral blood mononuclear cells (PBMCs) of patients with systemic juvenile idiopathic arthritis (sJIA), establish correlation between disease activity, laboratory parameters cytokines. The mRNA PBMCs concentrations 46 sJIA 30 age- sex-matched healthy controls were measured by real-time polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent assay (ELISA), respectively. correlations cytokines analyzed Spearman test. from stimulated recombinant human (rhIL-37) protein, expressions IL-1β, IL-6, TNF-α IL-17 detected RT-PCR ELISA. Plasma relative expression significantly elevated patients, especially active when compared (P < 0.001). Furthermore, showed higher mRNAs protein than those inactive well controls. correlated activity (IL-6, TNF-α, GM-CSF) patients. productions such obviously decreased after stimulation, whereas production IL-1β was not changed. Our results demonstrate which related In addition, rhIL-37 down-regulates form suggesting may have potential natural inhibitor for pathogenesis therapy sJIA.