Comprehensive analysis of aberrantly expressed profiles of mRNA and its relationship with serum galactose-deficient IgA1 level in IgA nephropathy

• DOI: 10.1186/s12967-019-2064-3
• Publication Date: 2019-09-23T13:02:42Z
• Authors: Youxia Liu; Xiangchun Liu; Junya Jia; Jie Zheng; Tiekun Yan
• Keywords: Adult; Male; 0301 basic medicine; Galactose-deficient IgA1; 03 medical and health sciences; RNA-deep sequencing; Humans; RNA, Messenger; Differential gene expression; Research; Gene Expression Profiling; R; PTEN Phosphohydrolase; Galactose; Reproducibility of Results; Glomerulonephritis, IGA; IgA nephropathy; Immunoglobulin A; 3. Good health; Gene Ontology; Gene Expression Regulation; Case-Control Studies; mRNA microarray; Medicine; Female

Abstract Background Immunoglobulin A nephropathy (IgAN) is the leading cause of end-stage kidney disease. Previous mRNA microarray profiling studies IgAN revealed inconsistent data. We sought to identify aberrantly expressed genes and biological pathways by integrating gene expression datasets in blood cells performing systematically experimental validation. also explored relationship between target galactose-deficient IgA1 (Gd-IgA1) IgAN. Methods retrieved Gene Expression Omnibus (GEO) Ontology (GO) enrichment Kyoto Encyclopedia Genes Genomes (KEGG) analyses were used for functional analysis. Deep sequencing on RNA isolated from B was The expressions Gd-IgA1 levels serum studied. Results Three with met our inclusion criteria. identified 655 dyregulated genes, including 319 up-regulated 336 down-regulated three GEO a total 35 patients 19 healthy controls. Based process GO term, these are mainly related pentose-phosphate shunt, non-oxidative branch, post-embryonic camera-type eye development leukocyte activation. KEGG pathway analysis data that enriched human T-cell leukemia virus 1 infection, proteoglycans cancer, intestinal immune network IgA production autophagy. further performed deep mRNAs an independent set five persons same clinical demographic characteristics. Seventy-seven overlapped differentially regulated mentioned above, 43 thirty-four genes. next investigated whether correlated patients. Pearson correlation showed PTEN (phosphatase tensin homolog) most powerful negatively levels. Conclusions These results demonstrated autophagy process, might be involved mechanism production.