Sulfur amino acid restriction, energy metabolism and obesity: a study protocol of an 8-week randomized controlled dietary intervention with whole foods and amino acid supplements

• DOI: 10.1186/s12967-021-02824-3
• Publication Date: 2021-04-15T14:48:47Z
• Authors: Emma Stolt; Thomas Olsen; Amany Elshorbagy; Viktor Kožich; Marleen van Greev...; Bente Øvrebø; Magne Thoresen; Helga Refsum; Kjetil Retterstøl; Kathrine J. Vinknes
• Keywords: Adult; Male; 0301 basic medicine; Adolescent; Metabolic health; 610; Adipose tissue; Sulfur amino acids; METHIONINE RESTRICTION; Young Adult; 03 medical and health sciences; 0302 clinical medicine; Protocol; Humans; Obesity; Amino Acids; Plasma biomarkers; Randomized Controlled Trials as Topic; RISK; 2. Zero hunger; PLASMA; R; Translational research; Middle Aged; PREVENTION; 3. Good health; Dietary intervention; Amino Acids, Sulfur; ADIPOSE-TISSUE; VDP::Medisinske Fag: 700::Helsefag: 800; Cysteine restriction; CYSTEINE; Body Composition; Medicine; LIFE-STYLE; Female; Gene expression; Energy Metabolism; Methionine restriction

Abstract Background Dietary sulfur amino acid (SAA) restriction is an established animal model for increasing lifespan and improving metabolic health. Data from human studies are limited. In the study outlined in this protocol, we will evaluate if dietary SAA can reduce body weight improve resting energy expenditure (REE) parameters related to Method/design Men women (calculated sample size = 60), aged 18–45 years, with mass index of 27–35 kg/m 2 be included a double-blind 8-week intervention study. The participants randomized 1:1 manner diet either low or high SAA. Both groups receive equal base consisting low-SAA plant-based whole foods supplement free Contrasting contents achieved using capsules without methionine cysteine (SAA , total ~ 50–60 mg/kg weight/day; 15–25 weight/day). primary outcome change. material collection also include composition (dual X-ray absorptiometry), (whole-room indirect calorimetry) samples blood, urine, feces adipose tissue at baseline, 4 weeks completion. Measures taken promote monitor adherence. analyzed linear mixed regression account repeated measures design within-subject correlation. Discussion strength design. A limitation restrictive nature which may lead poor compliance. If reveals beneficial effect on health, it opens up new strategies prevention treatment overweight, obesity its associated disorders. Trial registration ClinicalTrials.gov: NCT04701346, Registration date: January 8th, 2021