Study effect of probiotics and prebiotics on treatment of OVA-LPS-induced of allergic asthma inflammation and pneumonia by regulating the TLR4/NF-kB signaling pathway

• DOI: 10.1186/s12967-022-03337-3
• Publication Date: 2022-03-16T11:03:50Z
• Authors: Zhiwei Wu; Entezar Mehrabi N...; Poonam Arora; Seyyed Shamsadin ...
• Keywords: Allergic Inflammation; CCL11

Abstract Asthma is a common respiratory disease, and immune system dysregulation has direct relevance to asthma pathogenesis. Probiotics prebiotics have immunomodulatory effects can regulate responses may attenuate allergic reactions. Therefore, in this study, we explored the role of probiotics regulating acute airway inflammation TLR4/NF-kB pathway. Allergic model BALB/c mice was produced treated with (LA-5, GG, BB-12) (FOS GOS). Then AHR, BALF cells count, EPO activity, IL-4, 5, 13, 17, 25, 33, as well IFN-γ, total OVA-specific IgE, IgG1, Cys-LT, LTB4, LTC4, TSLP levels were measured. Also, GTP/GOT assay performed gene expression Akt, NLR3, NF-kB, PI3K, MyD88, TLR4, CCL11, CCL24, MUC5a, Eotaxin, IL-38, IL-8 determined. Finally, lung histopathological features evaluated. Treatment could control eosinophil infiltration reduce immunoglobulins, IL-17, GTP also decrease mucus secretion, goblet cell hyperplasia, peribronchial perivascular also, activity. It TLR4 CCL11. On other hand, IL-38 increased by both probiotic prebiotic treatment. leukotrienes, AKT, NF-κB, MUC5a. The treatment PI3K expression. Both treatments had no significant effect on GOT, IL-8, eotaxin CCL24 induce tolerance allegro-inflammatory reactions alter conditions. modulate cellular humoral prevent disorders.