Urinary exosomal hsa_circ_0001250 as a novel diagnostic biomarker of idiopathic membranous nephropathy

• DOI: 10.1186/s12967-022-03784-y
• Publication Date: 2022-12-19T12:13:31Z
• Authors: Qianyu Li; Mingzhu Xu; Zhiping Zhang; Min Yin; Yucheng Zhang; Feng Liu
• Keywords: KEGG; Exosome

Abstract Aims Idiopathic membranous nephropathy (IMN) is a common cause of adult nephrotic syndrome. Currently, the diagnosis IMN mainly depends on renal biopsy, which invasive. What’s more, markers already known for clinical are not sensitive enough. The present study aims to investigate profiling urinary exosomal circular RNAs (circRNAs) IMN, and look potential biomarker IMN. Methods Urine exosomes were collected from patients with idiopathic syndrome (INS), as well healthy controls (HCs) by ultracentrifuge. A pairwise comparison between 5 HC was performed high-throughput sequencing. Enrichment analysis explore functions differentially expressed circRNAs in Among three may be involved signaling pathways pathogenesis matched conserved mouse circRNAs, hsa_circ_0001250 selected target circRNA after quantitative polymerase chain reaction among 23 19 INS 23HC. Sanger sequencing RNase R digestion assay validated ring-structure sequence hsa_circ_0001250. ROC (Receiver Operating Characteristic) curve correlation used further validate utility diagnostic circRNA-miRNA-mRNA network constructed reflect relationship its miRNAs mRNAs. Results 766 up-regulated 283 down-regulated identified patients. Kyoto Encyclopedia Genes Genomes pathway revealed different participate in. confirmed, expression significantly increased relevant high level proteinuria. reflected that play role hsa-miR-639 hsa-miR-4449. Conclusion We functional profile Urinary tested predicted constructed.