IL-33 regulates adipogenesis via Wnt/β-catenin/PPAR-γ signaling pathway in preadipocytes

• DOI: 10.1186/s12967-024-05180-0
• Publication Date: 2024-04-17T09:02:58Z
• Authors: Danning Xu; Siqi Zhuang; Hongzhi Chen; Mengjie Jiang; Ping Jiang; Qian Wang; Xuemei Wang; Ruohong Chen; Haoneng Tang; Lingli Tang
• Keywords: CD36; 3T3-L1

Abstract Interleukin-33 (IL-33), an emerging cytokine within the IL-1 family, assumes a pivotal function in control of obesity. However, specific mechanism its regulation obesity formation remains unclear. In this study, we found that expression level IL-33 increased visceral adipose tissue mice fed with high-fat diet (HFD) compared normal (ND). vitro, also was upregulated during adipogenesis 3T3-L1 cells. Functional test results showed knockdown cells differentiation could promote accumulation lipid droplets, content triglyceride and adipogenic–related genes (i.e. PPAR-γ, C/EBPα, FABP4, LPL, Adipoq CD36). contrast, overexpression inhibits adipogenic differentiation. Meanwhile, above tests were repeated after over-differentiation induced by oleic acid, played more significant role adipogenesis. To explore mechanism, transcriptome sequencing performed regulated PPAR signaling pathway Further, Western blot confocal microscopy inhibition PPAR-γ inhibiting Wnt/β-catenin signal This study demonstrated important regulator preadipocyte inhibited regulating Wnt/β-catenin/PPAR-γ pathway, which provided new insight for further research on as intervention target metabolic disorders.