IL-10 plays an important role in the control of inflammation but not in the bacterial burden in S. epidermidis CNS catheter infection

• DOI: 10.1186/s12974-016-0741-1
• Publication Date: 2016-10-13T13:11:35Z
• Authors: Yenis M. Gutierre...; Gwenn Skar; Danielle Ramirez; Matthew Beaver; Jessica N. Snowden
• Keywords: Ventriculitis

Shunt infection is a frequent and serious complication in the surgical treatment hydrocephalus. Previous studies have shown an attenuated immune response to these biofilm-mediated infections. We proposed that IL-10 reduces inflammatory Staphylococcus epidermidis (S. epidermidis) CNS catheter infection. In this study, murine model of catheter-associated S. biofilm was generated based on well-established similar for aureus. The catheters were pre-coated with clinically derived biofilm-forming strain (strain 1457) which then stereotactically implanted into lateral left ventricle 8-week-old C57BL/6 knockout (IL-10 knockout) mice. Bacterial titers as well cytokine chemokine levels measured at days 3, 5, 7, 10 mice sterile epidermidis-coated catheters. Cultures demonstrated parenchymal persisted through following Cytokine analysis tissue surrounding revealed greater IL-10, anti-inflammatory cytokine, infected group compared sterile. KO mice, we noted no change bacterial burdens, showing not needed control model. However, had increased pro-inflammatory mediators tissues immediately adjacent catheter, increase weight loss. Together our results indicate plays key role regulating but burdens. Therefore, may be useful therapeutic target modulation should used conjunction antibiotic therapy eradication.