Absence of the lectin-like domain of thrombomodulin reduces HSV-1 lethality of mice with increased microglia responses

• DOI: 10.1186/s12974-022-02426-w
• Publication Date: 2022-03-11T08:02:59Z
• Authors: Meng-Shan Tsai; Li-Chiu Wang; Hua-Lin Wu; Shun-Fen Tzeng; Edward M. Conway; Sheng-Min Hsu; Shun-Hua Chen
• Keywords: Mice, Knockout; 0301 basic medicine; Research; Thrombomodulin; Herpesvirus 1, Human; HSV-1; 3. Good health; Mice, Inbred C57BL; Lectin-like domain; Mice; 03 medical and health sciences; Lectins; Encephalitis; Animals; Neurology. Diseases of the nervous system; Microglia; RC346-429

Herpes simplex virus 1 (HSV-1) can induce fatal encephalitis. Cellular factors regulate the host immunity to affect severity of HSV-1 Recent reports focus on significance thrombomodulin (TM), especially domain 1, lectin-like (TM-LeD), which modulates immune responses bacterial infections and toxins various diseases in murine models. Few studies have investigated importance TM-LeD viral infections, are also regulated by immunity.In vivo comparing wild-type knockout mice were performed determine role lethality. In vitro using brain microglia cultured from or a human cell line investigate whether how affects reduce replication neurons neuronal line.Absence decreased mortality, tissue loads, neuron apoptosis HSV-1-infected with increases number, proliferation, phagocytic activity microglia. Moreover, deficiency enhanced line. Co-culture mouse primary lines revealed that augmented capacity neurons.Overall, suppresses enhance infection.