Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B

• DOI: 10.1186/s12985-017-0692-x
• Publication Date: 2017-02-21T13:00:16Z
• Authors: Natália Lucinda; Maria Marta Figue...; Natália Lima Pessoa; Beatriz Senra Álv...; Graciela Kunrath ...; Arthur Molinari F...; Alexandre Magalhã...; Erna Geessien Kroon; Lis Ribeiro do Va...; Marco Antônio Campos
• Keywords: Granzyme A; Granzyme

Herpes simplex virus type 1 (HSV-1) cause not only mild symptoms but also blindness and encephalitis. It was previously shown that the immune response against HSV-1 occurs mainly in trigeminal ganglia (TG) Toll-like receptors 2 9 (TLR2/9) are important mediating this response. demonstrated iNOS (nitric oxide synthase) interleukin beta (IL-1β) play an essential role defense infection. Importantly, present work aimed to identify primary cells responsible for IL-1β production search other molecules might or depend on TLR2/9 mediate HSV-1. C57BL/6 (wild type, WT) TLR2/9−/− mice were infected by intranasal route with (1 × 106 p.f.u.). Cells obtained from TG spleen tissues profile of determined flow cytometry mock WT knockout mice. The percentage producing iNOS, IL-1β, granzyme B perforin cytometry. Chemokine monocyte chemoattractant protein-1 (MCP1) measured Cytometric Bead Array (CBA) TG, lung. Expression I interferons (IFNs), interleukins (IL) 5 10, quantified real time PCR. results indicate dendritic (DCs) monocytes/macrophages (Mo/Mϕ) main sources respectively, which, together IFNs, Additionally, we showed produced CD8+ T NK lymphocytes MCP-1 Moreover, our data robust is either TLR-independent down regulated TLRs Taken together, provide strong evidence responses mediated DCs, Mo/Mϕ, through production, IFN early infection, crucial host