Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus

• DOI: 10.1186/s12985-017-0783-8
• Publication Date: 2017-06-19T14:23:21Z
• Authors: Jauharotus Shobahah; Shengjie Xue; Dongbing Hu; Cui Zhao; Ming Wei; Yanping Quan; Wei Yu
• Keywords: Bombyx; Baculoviridae

Bombyx mori has become an important model organism for many fundamental studies. nucleopolyhedrovirus (BmNPV) is a significant pathogen to mori, yet also efficient vector recombinant protein production. A previous study indicated that acetylation plays vital roles in several cellular processes of while global phosphorylation pattern upon BmNPV infection remains elusive. Employing tandem mass tag (TMT) labeling and affinity enrichment followed by high-resolution LC-MS/MS analysis intensive bioinformatics analysis, the quantitative phosphoproteome cells infected at 24 hpi with MOI 10 was extensively examined. Totally, 6480 sites 2112 groups were identified, among which 4764 1717 proteins quantified. Among quantified proteins, 81 up-regulated 25 down-regulated identified criteria (the ratio above 1.3 considered as up-regulation below 0.77 down-regulation) p-value (p < 0.05). Some hyperphosphorylated during infection, such P6.9, 39 K, LEF-6, Ac58-like protein, Ac82-like BRO-D. The phosphorylated primary involved specific functions, out which, we focused on binding activity, synthesis, viral replication apoptosis through kinase activity.