Identification and characterisation of Gamma-herpesviruses in zoo artiodactyla

• DOI: 10.1186/s12985-024-02311-3
• Publication Date: 2024-02-23T22:01:36Z
• Authors: Laura Bianchessi; Edmund Flach; Giulia Monacchia; Mark Dagleish; Madeleine Maley; Lauretta Turin; Mara Silvia Rocchi
• Keywords: 0303 health sciences; Artiodactyla; DNA polymeras; Herpesvirus; Malignant catarrhal fever; Pan-herpes consensus PCR; Phylogenetic analyses; Virus surveillance; DNA polymeras; Research; Malignant catarrhal fever; Herpesvirus; Infectious and parasitic diseases; RC109-216; Ruminants; Virus surveillance; Pan-herpes consensus PCR; 03 medical and health sciences; Malignant Catarrh; Animals; Cattle; Phylogeny; Herpesviridae; Artiodactyla

Abstract Background Viruses within the γ-herpesviruses subfamily include the causative agents of Malignant Catarrhal Fever (MCF) in several species of the order Artiodactyla. MCF is a usually fatal lymphoproliferative disease affecting non-adapted host species. In adapted host species these viruses become latent and recrudesce and transmit during times of stress or immunosuppression. The undetected presence of MCF-causing viruses (MCFVs) is a risk to non-adapted hosts, especially within non-sympatric zoological collections. This study investigated the presence of MCFVs in six different zoological collections in the UK, to evaluate the presence of subclinical/latent MCFVs in carrier animals.

Methods One-hundred and thirty eight samples belonging to 54 different species of Artiodactyla were tested by Consensus Pan-herpes PCR. The positive samples were sequenced and subjected to phylogenetic analyses to understand their own evolutionary relationships and those with their hosts.

Results Twenty-five samples from 18 different species tested positive. All viruses but one clustered in the γ-herpesvirus family and within the Macavirus as well as the non-Macavirus groups (caprinae and alcelaphinae/hippotraginae clusters, respectively). A strong association between virus and host species was evident in the Macavirus group and clustering within the caprinae group indicated potential pathogenicity.

Conclusion This study shows the presence of pathogenic and non-pathogenic MCFVs, as well as other γ-herpesviruses, in Artiodactyla species of conservation importance and allowed the identification of new herpesviruses in some non-adapted species.