Genotoxic and epigenotoxic effects in mice exposed to concentrated ambient fine particulate matter (PM2.5) from São Paulo city, Brazil

• DOI: 10.1186/s12989-018-0276-y
• Publication Date: 2018-10-19T10:34:25Z
• Authors: Antonio Anax Falc...; Tiago Franco de O...; Michelle Francini...; Marisa Helena Gen...; Paolo Di Mascio; Mariana Veras; Miriam Lemos; Tania Marcourakis; Paulo Hilário Nas...; Ana Paula Melo Lo...
• Keywords: Deoxyguanosine; Glutathione reductase

The Metropolitan Area of São Paulo has a unique composition atmospheric pollutants, and positive correlations between exposure the risk diseases mortality have been observed. Here we assessed effects ambient fine particulate matter (PM2.5) on genotoxic global DNA methylation hydroxymethylation changes, as well activities antioxidant enzymes, in tissues AJ mice exposed whole body to air enriched PM2.5, which was concentrated chamber near an avenue intense traffic City, Brazil. Mice PM2.5 (1 h daily, 3 months) were compared situ study control. group presented increased levels oxidized nucleoside 8-oxo-7,8-dihydro-2′-deoxyguanosine lung kidney etheno adducts 1,N6-etheno-2′-deoxyadenosine 1,N2-etheno-2′-deoxyguanosine liver DNA, respectively. Apart from effects, led decreased epigenetic mark 5-hydroxymethylcytosine (5-hmC) DNA. Changes lung, liver, erythrocyte enzyme also Decreased glutathione reductase superoxide dismutase (SOD) observed lungs, while S-transferase SOD activities. An increase activity erythrocytes. These changes are consistent with induction local systemic oxidative stress. daily at concentration that mimics 24-h mean found presented, after months, lesions related occurrence stress kidney, parallel 5-hmC Genetic alterations induced by pollutants may affect genes committed cell cycle control, apoptosis, differentiation, increasing chance cancer development, merits further investigation.