CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis

• DOI: 10.1186/s13018-021-02432-3
• Publication Date: 2021-05-08T05:06:36Z
• Authors: Xia Li; Meiting Qu; Jie Zhang; Kuanyin Chen; Xianghui Ma
• Keywords: 0301 basic medicine; Gene Expression; HIPK2; Diseases of the musculoskeletal system; Protein Serine-Threonine Kinases; Arthritis, Rheumatoid; 03 medical and health sciences; Cell Movement; Humans; Fibroblast-like synoviocytes; Cells, Cultured; Cell Proliferation; Orthopedic surgery; Inflammation; 0303 health sciences; circASH2L; Cell Cycle; Gene Expression Regulation, Developmental; Nuclear Proteins; RNA, Circular; Fibroblasts; Synoviocytes; DNA-Binding Proteins; MicroRNAs; miR-129-5p; RC925-935; Disease Progression; Carrier Proteins; RA; RD701-811; Research Article; Transcription Factors

Previous study showed that circular RNA Absent-Small-Homeotic-2--Like protein (circASH2L) was higher in rheumatoid arthritis (RA) patients. However, the roles and mechanisms of circASH2L RA progression remain unclear.Levels analysis conducted using western blot qRT-PCR. The proliferation, apoptosis, cell cycle progression, migration, invasiveness, inflammation fibroblast-like synoviocytes (RA-FLSs) were determined via MTT, flow cytometry, blot, transwell, ELISA assays.CircASH2L knockdown RA-FLSs suppressed proliferative, migratory, invasive capacities, triggered arrest, promoted inhibited inflammation. Mechanistically, targeted miR-129-5p, repression miR-129-5p abolished functions silencing on growth, motility, RA-FLSs. Besides, found to directly target HIPK2, tumor-like biologic behaviors regulating HIPK2. Importantly, we proved could modulate HIPK2 expression miR-129-5p.CircASH2L RA-FLS through miR-129-5p/HIPK2 axis.