Dynamic exacerbation in inflammation and oxidative stress during the formation of peritendinous adhesion resulted from acute tendon injury

• DOI: 10.1186/s13018-021-02445-y
• Publication Date: 2021-05-05T05:02:56Z
• Authors: Pengfei Li; Haiying Zhou; Tian Tu; Hui Lu
• Keywords: Male; 0301 basic medicine; Time Factors; Interleukin-1beta; Tissue Adhesions; Diseases of the musculoskeletal system; Achilles Tendon; Rats, Sprague-Dawley; Tendons; Transforming Growth Factor beta1; 03 medical and health sciences; Tendon Injuries; Dynamic exacerbation; Animals; Peritendinous adhesion; Tendon surgery; Orthopedic surgery; Inflammation; 0303 health sciences; Superoxide Dismutase; Tumor Necrosis Factor-alpha; 3. Good health; Oxidative Stress; Acute tendon injury; RC925-935; Disease Progression; Inflammation Mediators; Reactive oxygen species; Reactive Oxygen Species; RD701-811; Research Article

AbstractBackgroundPeritendinous adhesion is among the common complications after tendon injury. Numerous studies have been carried out to prevent its formation, including modifications of surgical procedures, postoperative cares, application of medicines, etc. This study dynamically monitored fluctuations of inflammation, state of oxidative stress, and histopathologic changes around injured tendon to provide theoretical basis for further exploration in mechanisms of peritendinous adhesion formation.MethodsEighteen mature Sprague-Dawley male rats were randomly allocated into 6 equal groups. Compared with control and sham group, every rat’s right hind Achilles tendon in experimental groups was cut and repaired by the modified Kessler technique. Besides control and sham group, samples of tendon margin and serum were collected at different time points after the surgery. Content of TNF-α, IL-1β, and TGF-β were assayed in harvested serum. Reactive oxygen species (ROS) were detected, expression levels of related genes (IL-1β, IL-6, SOD1, SOD2, COL1, HIF1A) were quantified by qPCR, and various histopathological evaluations were performed.ResultsIndicators (TNF-α, IL-1β, TGF-β1, ROS) were noticed to have a similar trend of significant rising 24 h after the surgery except TGF-β which was rising 72 h later. So were the expression trends of IL-1β, IL-6, SOD1, SOD2, and COL1. HIF1A, inversely correlated with SOD2, showed the progressive relief of regional tissue hypoxia. Histological evaluation showed the same tendency that fibrosis and inflammation were getting serious 48 h later after the surgery.ConclusionsInflammation, oxidative stress in injured tendon resulted from acute trauma, would be getting intense in 24 h. Peritendinous adhesion emerges and aggravates after 48 h. Thus, prompt efficient measures are advised to be taken after the injury as soon as possible.