Qing-dai powder promotes recovery of colitis by inhibiting inflammatory responses of colonic macrophages in dextran sulfate sodium-treated mice

• DOI: 10.1186/s13020-015-0061-x
• Publication Date: 2015-10-13T20:15:19Z
• Authors: Hai-Tao Xiao; Jiao Peng; Dong-Dong Hu; Cheng-Yuan Lin; Bin Du; Siu-Wai Tsang; Ze-si Lin; Xiao-Jun Zhang; Feng-Ping Lueng; Quan-Bin Han; Zhao-Xiang Bian
• Keywords: Alum; Proinflammatory cytokine; Sulfasalazine

Qing-dai powder (QDP), comprising Indigo naturalis (Qing-dai) and dried alum (Ku-fan), was used in Chinese medicine to treat the conditions associated with mucosal hemorrhage, such as ulcerative colitis (UC). This study aims investigate effects potential mechanism of QDP on dextran sulfate sodium (DSS)-induced acute mice examine regulatory macrophages.Seven- eight-week-old male C57BL/6 were challenged 2.0 % DSS drinking water for 5 days then colitic arbitrarily allocated into five groups (n = 10 each group). (0.77, 1.54 3.08 g/kg) sulfasalazine (SASP) (0.20 orally administered 7 days. The disease activity index determined by scores body weight loss, diarrhea rectal bleeding; histological signs damage analyzed H&E staining; myeloperoxidase measured colorimetric method, levels proinflammatory cytokines ELISA; changes macrophages colon immunohistochemistry (IHC) flow cytometry. Lipopolysaccharide (LPS)-induced RAW264.7 cells treated or without QDP, production TNF-α IL-6 protein molecules COX-2, iNOS, IкB-α Western blot.Oral administration at dosages g/kg significantly reduced day 12 (P < 0.001 P 0.0008 g/kg), shortening 0.012 g/kg, colonic 0.002 DSS-treated mice. Moreover, treatment (1.54 decreased DSS-induced infiltration macrophages, 0.005 IL-1β 0.008 0.011 0.004 tissues, also serum MCP-1 g/kg). In cells, suppressed LPS-induced (Both 1.0 μg/mL treatment) expression COX-2 1 3 treatment, respectively) iNOS inhibiting degradation 0.007 NF-кB p65 nuclear translocation.QDP inflammatory responses UC cells.