An intersectional analysis of LncRNAs and mRNAs reveals the potential therapeutic targets of Bi Zhong Xiao Decoction in collagen-induced arthritis rats

• DOI: 10.1186/s13020-022-00670-z
• Publication Date: 2022-09-16T02:03:34Z
• Authors: Cailin He; Yang Wang; Yuqi Wen; Teng Li; En Hu; Siqing Zeng; Bo Yang; Xingui Xiong
• Keywords: Decoction; KEGG

Abstract Background Bi Zhong Xiao decoction (BZXD), a traditional Chinese herbal formula, has been used clinically for many years to treat rheumatoid arthritis (RA). Both clinical and experimental studies have revealed that BZXD is effective in treating RA, but the mechanism remains unclear. In this study, we aimed explore of efficacy through transcriptomic analysis lncRNA mRNA. Methods The combination method ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry was assess quality BZXD. collagen-induced (CIA) evaluated by assessment, weight changes, hematoxylin–eosin safranin o-fast green staining, Micro-CT. Arraystar rat lncRNA-mRNA chip technology determine mRNA expression profiles Control, CIA groups, screen gene related curative effect A co-expression network constructed therapeutic genes. Through GO function KEGG pathway enrichment analysis, biological functions signaling pathways genes were determined. Based on fold change functional annotation, key differentially expressed lncRNAs mRNAs selected reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation. targeting verified vitro. Results We demonstrated could effectively bone erosion. After treatment, up 33 107 reversely regulated These are mainly involved process immune response closely ECM-receptor interaction, MAPK pathway, Focal adhesion, Ras Antigen processing presentation, Chemokine pathway. identified four (uc.361−, ENSRNOT00000092834, ENSRNOT00000089244, ENSRNOT00000084631) three (Acvr2a, Cbx2, Morc4) as potential targets their microarray data consistent with RT-qPCR. vitro experiments confirmed silencing ENSRNOT00000092834 ENSRNOT00000084631 reversed target mRNAs. Conclusions This study elucidates possible reversing erosion rats from perspective To provide basis direction further exploration RA.