Spinal microglia contribute to sustained inflammatory pain via amplifying neuronal activity

• DOI: 10.1186/s13041-022-00970-3
• Publication Date: 2022-10-26T17:02:47Z
• Authors: Nan Gu; Min-Hee Yi; Madhuvika Murugan; Manling Xie; Sebastian Parusel; Jiyun Peng; Ukpong B. Eyo; Christine L. Hunt; Hailong Dong; Long-Jun Wu
• Keywords: Neurons; 0303 health sciences; Inflammatory pain; Research; 12. Responsible consumption; Formalin; Mice; 03 medical and health sciences; Formaldehyde; Purinergic P2Y Receptor Antagonists; Animals; Neuralgia; Neurology. Diseases of the nervous system; Microglia; P2Y12 receptor; Two-photon imaging; RC346-429; Microglia–neuron interaction

Abstract Microglia are highly dynamic immune cells of the central nervous system (CNS). Microglial processes interact with neuronal elements constantly on order minutes. The functional significance this acute microglia-neuron interaction and its potential role in context pain is still largely unknown. Here, we found that spinal microglia increased their process motility electrophysiological reactivity within an hour after insult a mouse model formalin-induced acute, sustained, inflammatory pain. Using ablation strategy to specifically deplete resident CNS, demonstrate participate sustained behaviors by amplifying activity dorsal horn. Moreover, identified P2Y12 receptor, which expressed was required for microglial function Taken together, our study provides novel insight into contribution receptor could be used therapeutic strategies.