Long non-coding RNA UBE2CP3 enhances HCC cell secretion of VEGFA and promotes angiogenesis by activating ERK1/2/HIF-1α/VEGFA signalling in hepatocellular carcinoma

• DOI: 10.1186/s13046-018-0727-1
• Publication Date: 2018-06-04T10:53:53Z
• Authors: Jinduan Lin; Shunwang Cao; Yu Wang; Yanwei Hu; Hongwei Liu; Jiehua Li; Jing Chen; Pan Li; Jumei Liu; Qian Wang; Lei Zheng
• Keywords: CD31; Chorioallantoic membrane

Angiogenesis is considered as an important process in the development of malignancies and associated with cancer progression metastasis. Hepatocellular carcinoma (HCC) most common primary tumor liver recognized a typical angiogenic tumor. Thus, it great importance to study underlying mechanism angiogenesis HCC. The long non-coding RNA (lncRNA) ubiquitin conjugating enzyme E2C pseudogene 3 (UBE2CP3) has been reported oncogene that promotes metastasis However, role mechanisms UBE2CP3 HCC are still unclear. We measured expression levels by situ hybridization (ISH) quantitative real-time polymerase chain reaction (qRT-PCR) patient samples. also concomitantly used CD31/PAS double-staining measure endothelial vessel (EV) density qRT-PCR CD31 mRNA level. HepG2 SMMC-7721 cells were transfected Lv-UBE2CP3 or Sh-UBE2CP3 virus obtain stably over-expressing knocking-down cell lines. indirect effects on ECs studied establishing co-culture system using Transwell chambers 0.4-μm pore size. separated, but cytokines growth factors able communicate each other. Following exposed cells, collected for functional studies. Finally, we function vivo chick embryo chorioallantoic membrane (CAM) assays nude mouse tumorigenicity assays. In this study, found was higher tissues than para-tumor up-regulated high EV density. Functionally, systems, overexpressing promoted HUVEC proliferation, migration tube formation via activation ERK/HIF-1α/p70S6K/VEGFA signalling, increasing level VEGFA supernatant. addition, opposite results appeared when knocked down. Consistent these results, CAM showed vivo. Our suggests can enhance interaction between HUVECs promote facilitating angiogenesis.