Assessment of Prostaglandin-Endoperoxide Synthase 2 and Versican gene expression profile from the cumulus cells: association with better in vitro fertilization outcomes

• DOI: 10.1186/s13048-018-0456-2
• Publication Date: 2018-09-21T04:43:59Z
• Authors: Azucena Ocampo; Jeimy Pedraza; Ginna Ortiz; Elizabeth Hernánd...; Leonardo Porchia; Esther López-Bayghen
• Keywords: Versican

Current methods for determining superior embryo quality (morphological assessment) are unable to compensate poor pregnancy outcomes. Due the importance of cumulus-oocyte complex and value cumulus cells (CCs) as markers health, we determined association between CCs gene expression Prostaglandin-Endoperoxide Synthase 2 (PTGS2) Versican (VCAN) with pregnancy.One hundred forty-nine women, suffering from infertility undergoing IVF, were included in this study (age: 29-46 years; BMI = 25.5 ± 5.0 kg/m2). Patients underwent a standard IVF protocol. isolated during oocyte retrieval, their RNA was using Trizol. The mRNA PTGS2, VCAN, L19 measured by qPCR. PVL index, (PTGS2 + VCAN)*L19normalized, each oocyte. Clinical confirmed β-hCG presence fetal heartbeat. Associations ROC curves or logistic regression.There no correlation index morphological scores. Using only single transfers (SETs), that associated (β-hCG: AUC 0.87, 95%CI: 0.74-1.00) an optimal cutoff 58.2. complete cohort (consisting SETs, patients 2, 3, 4 embryos transferred), at least one score ≥ 58.2 greater probability achieving odds ratio 17.15, 6.82-43.18, p < 0.001).Transferring 58.2, generates higher chance pregnancy.