MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4

• DOI: 10.1186/s13048-020-00686-9
• Publication Date: 2020-08-09T18:02:47Z
• Authors: Ji Lin; Huijuan Huang; Liheng Lin; Weiwei Li; Jianfen Huang
• Keywords: CDC42

Abstract Background MiRNAs play important roles in the development of ovarian cancer, activation primitive follicles, follicular development, oocyte maturation and ovulation. In present study, we investigated specific role miR-23a cov434 cells. Results Downregulation was observed serum PCOS patients compared with healthy control, suggesting inhibitory effect PCOS. MiR-23a positively correlated Body Mass Index (BMI) negatively Luteinizing hormone (LH), Testostrone (T), Glucose (Glu) Insulin (INS) patients. mimic inhibited proliferation promoted apoptosis human addition, flow cytometry assay confirmed that blocked cell cycle on G0/G1 phase. inhibitor showed opposite results. Furthermore, double luciferase reporter proved could bind to 3’UTR FGD4 directly through sites predicted Target Scan. level significantly suppressed by mimic, but enhanced inhibitor. We further increased expression activated CDC42 (GTP bround) p-PAK-1, induced arrest CDC42/PAK1 pathway. Conclusions conclusion, our study reveals participates regulation cells target FGD4, may a pathophysiology