Genome-wide survey of cytochrome P450 genes in the salmon louse Lepeophtheirus salmonis (Krøyer, 1837)

• DOI: 10.1186/s13071-019-3808-x
• Publication Date: 2019-11-27T06:02:43Z
• Authors: Joseph L. Humble; Greta Carmona-Ant...; Carol M. McNair; David R. Nelson; David I. Bassett; Ingibjørg Egholm; James E. Bron; Michaël Bekaert; Armin Sturm
• Keywords: Lepeophtheirus; Xenobiotic

The salmon louse (Lepeophtheirus salmonis) infests farmed and wild salmonid fishes, causing considerable economic damage to the farming industry. Infestations of are controlled using a combination non-medicinal approaches veterinary drug treatments. While L. salmonis has developed resistance most available delousing agents, relatively little is known about molecular mechanisms involved. Members cytochrome P450 (CYP) superfamily typically monooxygenases, some which involved in biosynthesis metabolism endogenous compounds, while others have central roles detoxification xenobiotics. In terrestrial arthropods, insecticide can be based on enhanced expression CYPs. reported research aimed characterise CYP assess its potential resistance.Lepeophtheirus CYPs were identified by homology searches genome transcriptome parasite. transcript abundance susceptible multi-resistant was assessed quantitative reverse transcription PCR, taking into account both constitutive parasites exposed sublethal levels ecdysteroids environmental chemicals.The above strategy led identification 25 genes/pseudogenes salmonis, making compact characterised for any arthropod date. Lepeophtheirus possesses homologues number genes with ecdysteroid metabolism, such as fruit fly disembodied, shadow, shade, spook Cyp18a1. did not differ between one strain salmonis. Exposure emamectin benzoate or deltamethrin caused transcriptional upregulation certain contrast, neither nor benzo[a]pyrene exposure affected significantly.The parasite demonstrated possess complement includes conserved well drug-inducible genes. present study does provide evidence role decreased susceptibility multiresistant studied.