Chromatin organization changes during the establishment and maintenance of the postmitotic state

• DOI: 10.1186/s13072-017-0159-8
• Publication Date: 2017-11-10T12:20:40Z
• Authors: Yiqin Ma; Laura Buttitta
• Keywords: Human genetics

Genome organization changes during development as cells differentiate. Chromatin motion becomes increasingly constrained and heterochromatin clusters become restricted in their developmental potential. These coincide with slowing of the cell cycle, which can also influence chromatin dynamics. Terminal differentiation is often coupled permanent exit from existing data suggest a close relationship between repressive structure silencing cycle postmitotic cells. Heterochromatin clustering could contribute to stable gene repression maintain terminal or exit, but whether initiated by differentiation, changes, both unclear. Here we examine organization, exit. We focused our studies on Drosophila wing, where epithelial transition active proliferation state temporally controlled manner. find there are two stages G0 this tissue, flexible period be induced reenter under specific genetic manipulations call "robust," strongly refractory reentry. Compromising driving ectopic expression activators causes global disruption heterochromatin-associated histone modifications such H3K27 trimethylation H3K9 trimethylation, well associated repressors, Polycomb protein 1 (HP1). However, reversible. When enter robust state, even presence activity, restored. If bypassed, wing continue terminally differentiate, severely disrupted. Heterochromatin-dependent does not appear required for compromising methyltransferase Enhancer zeste, and/or HP1 cannot prevent face normally oncogenic activities. consequence rather than differentiation. heterochromatin-dependent disrupt