Deciphering chicken gut microbial dynamics based on high-throughput 16S rRNA metagenomics analyses

• DOI: 10.1186/s13099-015-0051-7
• Publication Date: 2015-02-25T09:32:21Z
• Authors: Mohd Asrore Mohd ...; Chin Chin Sieo; Chun Wie Chong; Han Ming Gan; Yin Wan Ho
• Keywords: Medical microbiology; Parasitology

Chicken gut microbiota has paramount roles in host performance, health and immunity. Understanding the topological difference microbial community composition is crucial to provide knowledge on functions of each members physiological maintenance host. The profiling chicken was commonly performed previously using culture-dependent early culture-independent methods which had limited coverage accuracy. Advances technology based next-generation sequencing (NGS), offers unparalleled depth determining dynamics. Thus, aim this study investigate ileal caecal development as aged, important for future effective modulation.Ileal contents broiler were extracted from 7, 14, 21 42-day old chicken. Genomic DNA then amplified V3 hyper-variable region 16S rRNA. Bioinformatics, ecological statistical analyses such Principal Coordinate Analysis (PCoA) mothur software plotted PRIMER 6. Additional predicted metagenomes through PICRUSt STAMP package Greengenes databases.A distinctive bacterial communities observed between ilea caeca aged (P < 0.001). more diverse comparison communities. potentially pathogenic bacteria Clostridium elevated population beneficial microbe Lactobacillus low at all intervals. On other hand, analysed, clear distinction gene pathways related nutrient absorption (e.g. sugar amino acid metabolism), proliferation colonization motility proteins, two-component system secretion system) caeca, respectively 0.05).The ilea. main functional differences two sites found be colonization. Based community, modulation with probiotics may benefit