Use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats

• DOI: 10.1186/s13099-016-0129-x
• Publication Date: 2016-11-05T12:33:52Z
• Authors: Claire Joly Condette; Bertin Elion Dzon; Farida Hamdad; Maurice Biendo; Véronique Bach; Hafida Khorsi-Cauet
• Keywords: Enterococcus faecalis

Human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. Although digestive system is first organ to come into contact food contaminants, very few data available impact low-dose exposure during utero postnatal periods intestinal bacterial translocation (BT). Previous studies have revealed that chlorpyrifos (CPF) associated dysbiosis contamination sterile organs. Here, molecular typing was used investigate rats exposed lactation. The translocated bacteria were profiled, CPF tolerance antibiotic resistance traits determined. A total 72 segments extra-intestinal organs obtained from 14 CPF-exposed rats. samples cultured isolate strains had tolerated treatment 1 or 5 mg CPF/kg bodyweight/day vivo. Strains identified using matrix-assisted laser desorption/ionization (MALDI) Biotyper. disk diffusion method determine susceptibility. isolates genotyped PCR assays for enterobacterial repetitive intergenic consensus sequence random amplification polymorphic DNA. Bacterial confirmed 7 31 (22.6 %) isolated sites. Overall, most prevalent Staphylococcus aureus (55.5 % isolates), Enterococcus faecalis (27.7 Bacillus cereus (9.8 %). S. displayed methicillin resistance. Seventy two phenotypically, seven (mainly aureus) by genotyping. Genotypically mainly observed found pesticide-exposed groups (6 out 7). BT tract colonized normally Our findings validate use assessment critical development.