The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection

• DOI: 10.1186/s13099-017-0192-y
• Publication Date: 2017-08-31T14:21:07Z
• Authors: Alexandros Ch Str...; Filip Sima; Patrick Ward; Mark Linton; Carmel Kelly; Laurette Pinkerton; Lavinia Stef; Ioan Pet; Tiberiu Iancu; Gratiela Pircalab...; Nicolae Corcioniv...
• Keywords: Cryptosporidium parvum; Ex vivo; Gentamicin protection assay

Cryptosporidium is a major cause of diarrhea worldwide in both humans and farm animals with no completely effective treatment available at present. In this study, we assessed the inhibitory effect different concentrations Auranta 3001 (0.1, 0.5 1%), novel natural feed supplement, on C. hominis parvum invasion human ileocecal adenocarcinoma (HCT-8), bovine primary cells HCT-8, intestinal biopsies. The supplement production pro-inflammatory cytokines IL-8 INF-γ, anti-inflammatory cytokine IL-10, expression CpSUB1 protease gene during infection was also by quantitative PCR (q-PCR). Transepithelial electrical resistance (TEER) employed to measure integrity tight junction dynamics culture models.Pre-treatment or oocysts significantly reduced invasiveness against HCT-8 dose dependent manner. most pronounced reduction parasites observed when present infection. Levels were cells, while levels INF-γ IL-10 showed opposite trends two cell lines presence 3001. expression, which mediates infection, suggesting that enzyme possible target 3001.Although, use mechanisms infect additive can attenuate entry biopsies thus provide an alternative method control cryptosporidiosis.