Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children

• DOI: 10.1186/s13099-018-0235-z
• Publication Date: 2018-02-22T08:24:44Z
• Authors: Piotr Jarocki; Marcin Podleśny; Mariusz Krawczyk; Agnieszka Glibowska; Jarosław Pawelec; Elwira Komoń-Janc...; Oleksandr Kholiav...; Michał Dworniczak; Zdzisław Targoński
• Keywords: 0303 health sciences; 03 medical and health sciences; Lactobacillus rhamnosus Pen; Probiotics; Genome sequence; Prophage; RC799-869; Diseases of the digestive system. Gastroenterology; CRISPR–Cas locus; Genome Report; 3. Good health

BACKGROUND: Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. RESULTS: Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified. CONCLUSIONS: Genomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0235-z) contains supplementary material, which is available to authorized users.