Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells

• DOI: 10.1186/s13287-015-0013-5
• Publication Date: 2015-03-16T09:34:59Z
• Authors: Francisca Alcayag...; Jimena Cuenca; Patricia Luz-Craw...; Carolina Aguila-Díaz; Ainoa Fernandez; Fernando E Figueroa; Maroun Khoury
• Keywords: Hematopoietic stem cell

Stem cells isolated from menstrual fluid (MenSCs) exhibit mesenchymal stem cell (MSCs)-like properties including multi-lineage differentiation capacity. Besides, has important advantages over other sources for the isolation of MSCs, ease access and repeated sampling in a noninvasive manner. Such attributes allow rapid culture MenSCs numbers that are sufficient therapeutical doses, at lower passages.In this study, we advance characterization MenSC populations comparison to bone marrow derived (BM-MSCs) with regards proliferation, lineage differentiation, migration potential, secretion profile angiogenic vitro matrigel plug assay mice. We additionally tested their ability support hematopoietic (HSC) expansion vitro.The phenotypic analysis revealed largely similar BM-MSCs exception higher expression adhesion molecule CD49a (alpha1-integrin). Furthermore, fibroblast colony forming units (CFU-F) yielded 2 4 fold frequency progenitors capacity was superior BM-MSCs. In addition, evidenced paracrine response hypoxic conditions as by vascular endothelial growth factor basic also improved effect conditioned media on cells. were able induce angiogenesis vivo. Thus, an 8-fold increase hemoglobin content observed implanted plugs containing compared Finally, demonstrated, first time, ex-vivo HSCs, since rates CD34+CD133+ population well early progenitor (CFU-GEMM) colonies BM source.We present evidence showing superiority respect several functional aspects, However, impact such use adult-derived regenerative3 medicine remains be clarified.